Primary isolation and serial passage of hepatitis A virus strains in primate cell cultures.

Evaluating of Iran Razi Khedmati Bovine Kidney (IRKHBK) Cell Line for Isolation and ‎Propagation of Viruses

Abstract Background and Aims: To identify and isolation of viruses a susceptible cell is need which can be primary or line. The use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. IRKHBK cell line was originated from primary cells of bovine kidney by continuous passage. The aim of this study was to introduce the IRKHBK cell ...

Development and Cytogenetic Characterization of a Continuous Bovine Kidney Cell Line (IRKHBK) and Evaluation its Susceptibility to some Viruses

In this syudy a continuous bovine kidney cell line derived from a primary bovine kidney cells was established for the first time in Iran. The cells were originating from two-day-old normal male calf of Holstein breed. The cell cultures were continuously passaged following complete proliferation of primary cells. The specific properties or characteristics of the cell were defined using cytogenet...

Biologic properties of dengue viruses following serial passage in primary dog kidney cells: studies at the University of Hawaii.

Serial passage at low dilution of seven different wild-type dengue (DEN) viruses into primary dog kidney (PDK) cell cultures placed selective pressure that resulted in the following changes from parental phenotype: smaller plaques in LLC-MK2 cells, absent plaque formation in green monkey kidney cells, lack of a cytopathic effect in LLC-MK2 cells, shut-off of virus replication at high temperatur...

Immune response of chickens to four lentogenic strains of Newcastle disease virus propagated in lamb kidney cell cultures

Isolation and Identification of Yeast Strains Capable of Producing Single Cell Protein from Whey in Co-Cultures with Saccharomyces cerevisiae

In this study, twenty-five whey samples collected from dairy industries in the city of Isfahan. The sampleswere cultured on malt extract broth (MEB) and yeast extract glucose chloramphenicol agar (YGCA) media.Eleven yeast strains (designated M1 to M11) were isolated from the culture. The strains were identified bytheir morphological and physiological properties. Betagalactosid...